I am also riding through WV and VA this weekend. That much highway riding on a Ninja 300 sounds unpleasant. Also, your highway trip will bypass some of the best motorcycle roads in the eastern part of the US. On a curvy back road, where you're moving around on the bike you won't get nearly as stiff or uncomfortable as you will droning along the highway. If it were me, I would leave a few hours early and take the fun route. http://www.motoroads.net/